|
|
发表于 2013-5-21 22:12:51
|
显示全部楼层
shaniu 发表于 2013-5-21 22:00
倪老师，如果要培养分离出来的调节性T细胞，要注意什么啊，成本是不是很可观啊 ...
我没做过体外培养Treg，但从下面这个小鼠Treg的体外培养protocol看，应该成本较贵。
A modified protocol adapted from Tang et al was used to expand regulatory T cells in vitro. Isolated CD4＋CD25＋ regulatory T cells were placed in culture at 2×106 cells/2 mL/well (24 well plate) in RPMI 1640 medium with 7% FBS, 100 U/mL penicillin, 100 g/mL streptomycin, 2 mM glutamine, 50 M 2-ME, 1 mM sodium pyruvate, 0.01 mM nonessential amino acids, and 10 mM HEPES. Anti-mouse CD3– and anti-mouse CD28–coated 4 um polystyrene magnetic beads (Dynal; Invitrogen, Carlsbad, CA) were added at a 1:1 ratio. Recombinant mouse IL-2 (20 ng/mL; R&D Systems, Minneapolis, MN) was added to each well. The cultures were monitored daily and maintained at 0.7 to 1×106 cells/mL. At the end of the seventh day in culture, a magnet was used to remove the beads.
＝＝＝＝＝＝＝＝＝＝＝＝
使用流式中文网资料阅读器在线阅读，免流星，内容与附件完全一样，且支持划词翻译（使用帮助）
（如图片无法正常显示请复制链接：http://www.flowcyto.cn/bbs/reade ... 9KX13PzsTGczXGHGdM8）
◢如确实需要下载附件，请见：
invitro_expanded_Treg.pdf
(666.41 KB, 下载次数: 7)
|
|
楼主: shaniu
