看了文献，文献中Much of the CFSE at this point is not stably incorporated within the cells and is lost from the cells during the next 24–48 h. As a result, the initial fluorescence intensity is extremely high and can bleed significantly into other flow cytometry detectors (particularly those set for detection of fluorescence at wavelengths close to CFSE, i.e., the PE detector). This should be taken into consideration if cells are to be analyzed or sorted by a flow cytometer within the first few hours after staining, which is not recommended. This problem can be reduced by decreasing the concentration of CFSE during labeling, but this will reduce the
number of detectable cell divisions. 提到刚染色后大部分CFSE不能稳定地并入细胞内，并在下一次细胞内丢失24–48小时。因此，CFSE初始荧光强度非常高。如果细胞要在染色后的最初几小时内通过流式细胞仪进行分析或分选。那CFSE染色的浓度是不是的在染色后24h上机检测，看看CFSE强度来确认？不太明白这个 |
楼主: niwanmao
发表于 2016-11-18 20:43:03
