莫名其妙的荧光阳性信号，也可能是荧光素非特异性结合

niwanmao

通常，我们谈及染色的非特异性阳性信号时，通过就是两个原因：死细胞、抗体与细胞上Fc受体非特异性结合，对应的解决方法就是：用死活鉴别染料（如低浓度PI染液或商品化死活鉴别染料）、用Fc Block阻断剂或BSA等。

然而，有时候FLOWER们会发现，即使我老老实实用了上面两个绝招，并且荧光补偿也调节得非常完美了，却仍然还是有莫名其妙的荧光阳性信号，怎么回事？

此时，你可能就得仔细看一下方案，是不是荧光素选错了，导致荧光素非特异性结合，这个问题可能大家都没去关注。

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看了这么多，大家应该知道怎么做了吧，根据自己的实验目的，设计好方案的配色才是第一步，死细胞的排除和Fc阻断，仍应成为常规。



参考文献：
An amazing source for odd questions on flow cytometry is the ‘Cytometry mailing list’ hosted by the Purdue University, which can be found under: https://lists.purdue.edu/mailman/listinfo/cytometry
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